Hotspot

trnT-GGU-psbD

LSC · 31544-32709 · 1166 bp

Back to Clerodendrum

Pi 0.0139

MarkerSeek score 61.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 37
pi: 0.0139
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1324-1330 bp	1.0	80.0
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1323-1329 bp	1.0	78.9
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1287-1293 bp	1.0	78.7
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	TCCCTCCGTAACCAGTCATC	50.0	55.0	57.9	58.5	1309-1315 bp	1.0	77.9
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.3	57.6	1286-1292 bp	1.0	77.5