Hotspot

atpH-atpI

LSC · 13923-14971 · 1049 bp

Back to Clerodendrum

Pi 0.0152

MarkerSeek score 62.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 34
pi: 0.0152
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.1	57.9	836-1134 bp	1.0	84.7
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	854-1152 bp	1.0	84.3
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.8	57.9	853-1151 bp	1.0	83.7
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCCGCAG	50.0	45.0	58.3	57.9	848-1146 bp	1.0	83.4
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.6	57.9	849-1147 bp	1.0	82.1