Hotspot

psbI-trnS-GCU

LSC · 8476-8591 · 116 bp

Back to Clerodendrum

Pi 0.0302

MarkerSeek score 66.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 7
pi: 0.0302
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGACGCGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.7	58.0	204-219 bp	1.0	83.7
psbI-trnS-GCU_p2	GTAATCCTGGACGCGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.7	58.0	205-220 bp	1.0	83.7
psbI-trnS-GCU_p3	GTAATCCTGGACGCGAAGAA	TTAGCTTGGGAGAGATGGCT	50.0	50.0	57.7	58.1	227-242 bp	1.0	82.2
psbI-trnS-GCU_p4	GTAATCCTGGACGCGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.7	58.4	202-217 bp	1.0	80.9
psbI-trnS-GCU_p5	GTAATCCTGGACGCGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.7	58.4	209-224 bp	1.0	80.5