Hotspot

trnH-GUG-psbA

LSC · 76-386 · 311 bp

Back to Clerodendrum

Pi 0.0733

MarkerSeek score 79.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 311
variable_sites: 44
pi: 0.0733
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GTCGTGGGTATCTGGTTCAC	55.0	55.0	58.1	58.0	527-589 bp	1.0	90.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CGTGGGTATCTGGTTCACTG	55.0	55.0	58.1	58.0	525-587 bp	1.0	90.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GTCGTGGGTATCTGGTTCAC	50.0	55.0	57.9	58.0	541-603 bp	1.0	90.1
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GTCGTGGGTATCTGGTTCAC	50.0	55.0	57.9	58.0	540-602 bp	1.0	90.1
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	977-1039 bp	1.0	87.2