Hotspot

ycf1

SSC · 123560-128983 · 5424 bp

Back to Cleomella

Pi 0.00314

MarkerSeek score 44.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5424
variable_sites: 17
pi: 0.00314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAAGTTGACAGGTTGGGGG	TGATGAATTCCACGCTCGAG	50.0	50.0	58.2	58.1	310 bp	1.0	78.5
ycf1_p2	AAAAGTTGACAGGTTGGGGG	GAATTCCACGCTCGAGCATA	50.0	50.0	58.2	58.1	306 bp	1.0	77.9
ycf1_p3	AAAAGTTGACAGGTTGGGGG	TCCACGCTCGAGCATACTAT	50.0	50.0	58.2	58.4	302 bp	1.0	75.9
ycf1_p4	AAAAGTTGACAGGTTGGGGG	AATTCCACGCTCGAGCATAC	50.0	50.0	58.2	58.4	305 bp	1.0	75.7
ycf1_p5	AAAAGTTGACAGGTTGGGGG	ATGATGAATTCCACGCTCGA	50.0	45.0	58.2	57.4	311 bp	1.0	74.3