Hotspot

psbG

LSC · 48972-49649 · 678 bp

Back to Cleomella

Pi 0.00295

MarkerSeek score 46.10

Primer available yes

Label psbG

Feature Metadata

label_name: psbG
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 2
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG_p1	TCCGCTTGATTGACACCAAA	TGCCAATCCACTCTGATTCG	45.0	50.0	58.0	58.0	1516-1519 bp	1.0	79.8
psbG_p2	TTCCGCTTGATTGACACCAA	TGCCAATCCACTCTGATTCG	45.0	50.0	58.0	58.0	1517-1520 bp	1.0	79.8
psbG_p3	CACCCTATCCAACTTTCGGG	TGCCAATCCACTCTGATTCG	55.0	50.0	58.0	58.0	1690-1693 bp	1.0	79.7
psbG_p4	CCACCCTATCCAACTTTCGG	TGCCAATCCACTCTGATTCG	55.0	50.0	58.0	58.0	1691-1694 bp	1.0	79.7
psbG_p5	TCCGCTTGATTGACACCAAA	TTAGGAAGGGGCCAGAGAAA	45.0	50.0	58.0	57.9	1346-1349 bp	1.0	79.7