Hotspot

clpP

LSC · 69204-71289 · 2086 bp

Back to Cleomella

Pi 0.0024

MarkerSeek score 51.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2086
variable_sites: 5
pi: 0.0024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGATTTGTATGAAATCACTCCGA	CCCCATTTTTACGTTTCCACA	34.8	42.9	55.8	57.0	2174-2176 bp	1.0	40.3
clpP_p2	AGATTTGTATGAAATCACTCCGA	ACCCCATTTTTACGTTTCCAC	34.8	42.9	55.8	57.0	2175-2177 bp	1.0	40.3
clpP_p3	AGATTTGTATGAAATCACTCCGA	AACCCCATTTTTACGTTTCCAC	34.8	40.9	55.8	57.7	2176-2178 bp	1.0	40.3
clpP_p4	GATTTGTATGAAATCACTCCGA	CCCCATTTTTACGTTTCCACA	36.4	42.9	54.3	57.0	2173-2175 bp	1.0	40.3
clpP_p5	GATTTGTATGAAATCACTCCGA	ACCCCATTTTTACGTTTCCAC	36.4	42.9	54.3	57.0	2174-2176 bp	1.0	40.3