Hotspot

ndhD-psaC

SSC · 116745-116882 · 138 bp

Back to Cleomella

Pi 0.0543

MarkerSeek score 80.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 138
variable_sites: 7
pi: 0.0543
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGAACCCGCAGATATTGGAA	AAACAACTCGAAGCATGGGT	45.0	45.0	56.9	58.0	245 bp	1.0	76.3
ndhD-psaC_p2	TGAACCCGCAGATATTGGAA	GGCATGAAACAACTCGAAGC	45.0	50.0	56.9	58.0	251 bp	1.0	76.1
ndhD-psaC_p3	ATGAACCCGCAGATATTGGA	AAACAACTCGAAGCATGGGT	45.0	45.0	56.7	58.0	246 bp	1.0	74.5
ndhD-psaC_p4	ATGAACCCGCAGATATTGGA	GGCATGAAACAACTCGAAGC	45.0	50.0	56.7	58.0	252 bp	1.0	74.2
ndhD-psaC_p5	TGAACCCGCAGATATTGGAAA	AAACAACTCGAAGCATGGGT	42.9	45.0	57.6	58.0	245 bp	1.0	74.2