Hotspot

rpl32-trnL-CUA

SSC · 112861-113797 · 937 bp

Back to Cleomella

Pi 0.0128

MarkerSeek score 52.50

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 937
variable_sites: 12
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	AGCGTTGAAAGCTTTTTCGT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	57.2	57.9	1053-1055 bp	1.0	73.4
rpl32-trnL-CUA_p2	AGCGTTGAAAGCTTTTTCGT	AGCAGCGTGTCTACCAATTT	40.0	45.0	57.2	57.8	1046-1048 bp	1.0	72.9
rpl32-trnL-CUA_p3	AGCGTTGAAAGCTTTTTCGT	AACCACTGTGATTGGAGAGC	40.0	50.0	57.2	57.8	1621-1623 bp	1.0	72.6
rpl32-trnL-CUA_p4	AGCGTTGAAAGCTTTTTCGT	TAAAACACCCGAAGTCGCAA	40.0	45.0	57.2	58.0	1570 bp	0.5	52.9
rpl32-trnL-CUA_p5	AGCGTTGAAAGCTTTTTCGT	TTAAAACACCCGAAGTCGCA	40.0	45.0	57.2	58.0	1571 bp	0.5	52.9