Hotspot

rpl22-rps19

LSC · 83479-83532 · 54 bp

Back to Cleomella

Pi 0.0185

MarkerSeek score 63.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 54
variable_sites: 1
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAGGAACGTCCACGAATT	ATAAATTTCCGGGGGCATGC	50.0	50.0	58.0	59.0	217 bp	1.0	72.6
rpl22-rps19_p2	GCTTTGTGGGCAGACATAGA	ATAAATTTCCGGGGGCATGC	50.0	50.0	57.9	59.0	179 bp	1.0	71.9
rpl22-rps19_p3	TGCTTTGTGGGCAGACATAG	ATAAATTTCCGGGGGCATGC	50.0	50.0	58.2	59.0	180 bp	1.0	71.4
rpl22-rps19_p4	CGTAGGAACGTCCACGAATT	GGGGCATGCGAAAAATGATA	50.0	45.0	58.0	56.8	206 bp	1.0	70.9
rpl22-rps19_p5	GCTTTGTGGGCAGACATAGA	GGGGCATGCGAAAAATGATA	50.0	45.0	57.9	56.8	168 bp	1.0	70.2