Hotspot

rps12-clpP

LSC · 69010-69203 · 194 bp

Back to Cleomella

Pi 0.0361

MarkerSeek score 73.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 7
pi: 0.0361
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACATTTCGGATTGGCTGT	GTTGACCTTGTAGCGGTTCA	45.0	50.0	57.8	58.1	273 bp	1.0	79.5
rps12-clpP_p2	TGACATTTCGGATTGGCTGT	TGTTGACCTTGTAGCGGTTC	45.0	50.0	57.8	58.1	274 bp	1.0	79.4
rps12-clpP_p3	TGACATTTCGGATTGGCTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.8	57.9	307 bp	1.0	79.3
rps12-clpP_p4	TTTCGGATTGGCTGTCTTGT	GTTGACCTTGTAGCGGTTCA	45.0	50.0	57.7	58.1	268 bp	1.0	78.9
rps12-clpP_p5	TTTCGGATTGGCTGTCTTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.7	57.9	302 bp	1.0	78.8