Hotspot

ndhC-trnV-GUA

LSC · 50065-50767 · 703 bp

Back to Cleomella

Pi 0.00427

MarkerSeek score 61.20

Primer available yes

Label ndhC-trnV-GUA

Feature Metadata

label_name: ndhC-trnV-GUA
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 3
pi: 0.00427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-GUA_p1	TTTCTCTGGCCCCTTCCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	856-870 bp	1.0	78.3
ndhC-trnV-GUA_p2	TCTCTGGCCCCTTCCTAATT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	854-868 bp	1.0	76.4
ndhC-trnV-GUA_p3	TTCTCTGGCCCCTTCCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	855-869 bp	1.0	76.4
ndhC-trnV-GUA_p4	CTCTGGCCCCTTCCTAATTG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.6	57.8	853-867 bp	1.0	75.9
ndhC-trnV-GUA_p5	TTTCTCTGGCCCCTTCCTAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	855-869 bp	1.0	75.5