Hotspot

psbG-ndhC

LSC · 49650-49701 · 52 bp

Back to Cleomella

Pi 0.0385

MarkerSeek score 74.60

Primer available yes

Label psbG-ndhC

Feature Metadata

label_name: psbG-ndhC
feature_type: igs
region: LSC
length_bp: 52
variable_sites: 2
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG-ndhC_p1	GGCTGGAAAGTCTTGACCAA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	184-188 bp	1.0	80.0
psbG-ndhC_p2	TTTTGGTTGGTCGGTCAAGT	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.9	58.1	127-131 bp	1.0	79.5
psbG-ndhC_p3	GGCTGGAAAGTCTTGACCAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	272-276 bp	1.0	78.8
psbG-ndhC_p4	TTGGTTGGTCGGTCAAGTAC	CATGGCGAAAAGGAGCATTG	50.0	50.0	57.8	58.1	125-129 bp	1.0	78.6
psbG-ndhC_p5	TTTTGGTTGGTCGGTCAAGT	TGGGCAATGAGTTTCGATGT	45.0	45.0	57.9	57.8	215-219 bp	1.0	78.1