Hotspot

psbM-trnD-GAC

LSC · 29000-29945 · 946 bp

Back to Cleomella

Pi 0.00636
MarkerSeek score 55.30
Primer available yes
Label psbM-trnD-GAC

Feature Metadata

label_name
psbM-trnD-GAC
feature_type
igs
region
LSC
length_bp
946
variable_sites
6
pi
0.00636
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GAC_p1 TGAGAATGAAGAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.5 50.0 58.1 58.5 1058-1059 bp 1.0 59.6
psbM-trnD-GAC_p2 GAGAATGAAGAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 47.6 50.0 56.4 58.5 1057-1058 bp 1.0 56.3
psbM-trnD-GAC_p3 AGGAATGAGAATGAAGAGTGCA TTCAATTGGTCAGAGCACCG 40.9 50.0 57.4 58.5 1063-1064 bp 1.0 56.3
psbM-trnD-GAC_p4 TGAGAATGAAGAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 45.5 50.0 58.1 57.0 1059-1060 bp 1.0 55.5
psbM-trnD-GAC_p5 AGAATGAAGAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.2 58.5 1056-1057 bp 1.0 54.2