Hotspot

atpA-atpF

LSC · 11593-11656 · 64 bp

Back to Cleomella

Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CACTTGAAGTACGGTACCGG	ACAAGGAGCTATAGGAACCCT	55.0	47.6	58.0	57.6	278 bp	1.0	68.8
atpA-atpF_p2	CCACTTGAAGTACGGTACCG	ACAAGGAGCTATAGGAACCCT	55.0	47.6	58.0	57.6	279 bp	1.0	68.8
atpA-atpF_p3	GCCCACTTGAAGTACGGTAC	ACAAGGAGCTATAGGAACCCT	55.0	47.6	58.3	57.6	281 bp	1.0	66.6
atpA-atpF_p4	GATACCATCGCCCACTTGAA	ACAAGGAGCTATAGGAACCCT	50.0	47.6	57.7	57.6	290 bp	1.0	66.3
atpA-atpF_p5	ATACCATCGCCCACTTGAAG	ACAAGGAGCTATAGGAACCCT	50.0	47.6	57.7	57.6	289 bp	1.0	66.2