Hotspot

trnR-AGA-atpA

LSC · 9781-10068 · 288 bp

Back to Cleomella

Pi 0.0316

MarkerSeek score 71.60

Primer available yes

Label trnR-AGA-atpA

Feature Metadata

label_name: trnR-AGA-atpA
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 9
pi: 0.0316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-AGA-atpA_p1	GGTTCAAATCCTATTGGACGC	TCACCGAAGAAGCAGAAAGC	47.6	50.0	57.3	58.5	387-390 bp	1.0	65.0
trnR-AGA-atpA_p2	GGTTCAAATCCTATTGGACGC	ACATTCACCGAAGAAGCAGA	47.6	45.0	57.3	57.2	391-394 bp	1.0	62.2
trnR-AGA-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTCACCGAAGAAGCA	47.6	45.0	57.3	57.2	393-396 bp	1.0	62.2
trnR-AGA-atpA_p4	TGGATAGGACAGAGGTCTTCT	TCACCGAAGAAGCAGAAAGC	47.6	50.0	56.9	58.5	422-425 bp	1.0	62.0
trnR-AGA-atpA_p5	GGTTCAAATCCTATTGGACGC	AGAGCAACTGGAACGTTTTCT	47.6	42.9	57.3	58.1	347-350 bp	1.0	60.6