Hotspot

psbA-trnK-AAA

LSC · 1518-1792 · 275 bp

Back to Cleomella

Pi 0.00727

MarkerSeek score 53.70

Primer available yes

Label psbA-trnK-AAA

Feature Metadata

label_name: psbA-trnK-AAA
feature_type: igs
region: LSC
length_bp: 275
variable_sites: 2
pi: 0.00727
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-AAA_p1	TTTTCCGTGCTGGTTATCCA	CGGTTTGGGGAGGGATTTTT	45.0	50.0	57.4	58.4	445 bp	1.0	72.9
psbA-trnK-AAA_p2	TACAGAAGCGACCCCATAGG	CGGTTTGGGGAGGGATTTTT	55.0	50.0	58.6	58.4	423 bp	1.0	72.8
psbA-trnK-AAA_p3	TTTTCCGTGCTGGTTATCCA	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.4	58.4	457 bp	1.0	72.4
psbA-trnK-AAA_p4	TACAGAAGCGACCCCATAGG	AAAATGCAAGCACGGTTTGG	55.0	45.0	58.6	58.4	435 bp	1.0	72.3
psbA-trnK-AAA_p5	ATCCAGTTACAGAAGCGACC	CGGTTTGGGGAGGGATTTTT	50.0	50.0	57.3	58.4	430 bp	1.0	72.1