Hotspot

ycf1

SSC · 112279-117747 · 5469 bp

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Pi 0.342
MarkerSeek score 76.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5469
variable_sites
1697
pi
0.342
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AAGATTGAGTCCTGGGTCGA CAGACAGACGAACAGGTTCA 50.0 50.0 58.1 57.5 781 bp 0.5 55.4
ycf1_p2 AAGATTGAGTCCTGGGTCGA ACAGACAGACGAACAGGTTC 50.0 50.0 58.1 57.5 782 bp 0.5 55.4
ycf1_p3 GAAGGGTCTTGCCAATGACA CAGACAGACGAACAGGTTCA 50.0 50.0 58.1 57.5 695 bp 0.5 55.2
ycf1_p4 GAAGGGTCTTGCCAATGACA ACAGACAGACGAACAGGTTC 50.0 50.0 58.1 57.5 696 bp 0.5 55.2
ycf1_p5 GACGGGGTATAGGGGAAAGA CAGACAGACGAACAGGTTCA 55.0 50.0 57.9 57.5 849 bp 0.5 55.2