Pi
0.481
MarkerSeek score
79.50
Primer available
yes
Label
ndhH
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhH_p1 | GAAAACGTCAACGACTGCTG | ACTAATCGATTGGGCAGCAG | 50.0 | 50.0 | 58.0 | 58.0 | 1896 bp | 0.5 | 59.6 |
| ndhH_p2 | GAAAACGTCAACGACTGCTG | CCGTCTGCTAGAGCTTGAAG | 50.0 | 55.0 | 58.0 | 58.1 | 1622 bp | 0.5 | 59.3 |
| ndhH_p3 | GAAAACGTCAACGACTGCTG | CGTTGTTGTATCCCTGCAGA | 50.0 | 50.0 | 58.0 | 57.9 | 1568 bp | 0.5 | 59.2 |
| ndhH_p4 | GAAAACGTCAACGACTGCTG | ACTGCGAATTTCTAAGCCGT | 50.0 | 45.0 | 58.0 | 57.9 | 2101 bp | 0.5 | 59.2 |
| ndhH_p5 | GAAAACGTCAACGACTGCTG | CACGAATCGCACGTAGAGAT | 50.0 | 50.0 | 58.0 | 57.9 | 1951 bp | 0.5 | 59.1 |