Hotspot

ndhG

SSC · 122984-123514 · 531 bp

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Pi 0.453
MarkerSeek score 77.40
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
188
pi
0.453
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.78
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 TCTGCCTGTTGTTGATTGGA TCGATTGGTTGATGCGAGTT 45.0 45.0 57.3 57.9 1792 bp 0.5 54.0
ndhG_p2 TCTGCCTGTTGTTGATTGGA CGATTGGTTGATGCGAGTTG 45.0 50.0 57.3 57.8 1791 bp 0.5 53.4
ndhG_p3 TCTGCCTGTTGTTGATTGGA GCGGCTGCAATTGCTATAAC 45.0 50.0 57.3 58.2 1718 bp 0.5 52.8
ndhG_p4 TCTGCCTGTTGTTGATTGGA TCCAATAGTTGCTTCAGCGG 45.0 50.0 57.3 58.3 1734 bp 0.5 52.7
ndhG_p5 TCTGCCTGTTGTTGATTGGA AGCGGCTGCAATTGCTATAA 45.0 45.0 57.3 58.3 1719 bp 0.5 52.2