Hotspot

rpl20-rps12

LSC · 71716-72513 · 798 bp

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Pi 0.0439
MarkerSeek score 80.90
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
798
variable_sites
34
pi
0.0439
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATATCCGCGT ACAGCCAATCCGAAATGTCA 50.0 45.0 58.0 57.8 910-911 bp 1.0 83.1
rpl20-rps12_p2 TCCGAGCTATATATCCGCGT ACAAGACAGCCAATCCGAAA 50.0 45.0 58.0 57.7 915-916 bp 1.0 82.5
rpl20-rps12_p3 TCCGAGCTATATATCCGCGT AGACAGCCAATCCGAAATGT 50.0 45.0 58.0 57.5 912-913 bp 1.0 80.7
rpl20-rps12_p4 TTTGTTCTACGTCTCCGAGC ACAGCCAATCCGAAATGTCA 50.0 45.0 57.7 57.8 923-924 bp 1.0 80.6
rpl20-rps12_p5 GTAAGTCGTGAATGAGCCCC ACAGCCAATCCGAAATGTCA 55.0 45.0 58.3 57.8 971-972 bp 1.0 80.3