Hotspot

ycf4-cemA

LSC · 62569-63275 · 707 bp

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Pi 0.0808
MarkerSeek score 81.60
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
707
variable_sites
51
pi
0.0808
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCTTACTTCTTGCGTGT AGGGCAAAAAGACTATAGATGCA 45.0 39.1 58.0 57.9 747-807 bp 1.0 64.0
ycf4-cemA_p2 TTGGCTTACTTCTTGCGTGT CAGGGCAAAAAGACTATAGATGC 45.0 43.5 58.0 57.6 748-808 bp 1.0 61.5
ycf4-cemA_p3 TTGGCTTACTTCTTGCGTGT GGGCAAAAAGACTATAGATGCA 45.0 40.9 58.0 56.5 746-806 bp 1.0 60.8
ycf4-cemA_p4 TTGGCTTACTTCTTGCGTGT AGGGCAAAAAGACTATAGATGC 45.0 40.9 58.0 56.2 747-807 bp 1.0 58.6
ycf4-cemA_p5 TTGGCTTACTTCTTGCGTGT GGGCAAAAAGACTATAGATGCAA 45.0 39.1 58.0 57.2 746-806 bp 1.0 58.3