Hotspot

trnE-UUC-trnT-GGU

LSC · 32126-32889 · 764 bp

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Pi 0.0556
MarkerSeek score 81.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
764
variable_sites
41
pi
0.0556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 867-871 bp 1.0 84.0
trnE-UUC-trnT-GGU_p2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 846-850 bp 1.0 84.0
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 846-850 bp 1.0 83.7
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 825-829 bp 1.0 83.7
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 843-847 bp 1.0 83.5