Hotspot

trnS-GCU-trnT-CGU

LSC · 8634-9284 · 651 bp

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Pi 0.0694
MarkerSeek score 82.30
Primer available yes
Label trnS-GCU-trnT-CGU

Feature Metadata

label_name
trnS-GCU-trnT-CGU
feature_type
igs
region
LSC
length_bp
651
variable_sites
43
pi
0.0694
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-CGU_p1 CCAACGCTTTAGTCCACTCA GGAACGAATCACACTTTTACCA 50.0 40.9 57.8 56.9 719-953 bp 1.0 61.1
trnS-GCU-trnT-CGU_p2 AACGCTTTAGTCCACTCAGC GGAACGAATCACACTTTTACCA 50.0 40.9 58.2 56.9 717-951 bp 1.0 60.8
trnS-GCU-trnT-CGU_p3 CCAACGCTTTAGTCCACTCA GGAACGAATCACACTTTTACCAC 50.0 43.5 57.8 58.2 719-953 bp 1.0 60.0
trnS-GCU-trnT-CGU_p4 AACGCTTTAGTCCACTCAGC GGAACGAATCACACTTTTACCAC 50.0 43.5 58.2 58.2 717-951 bp 1.0 59.7
trnS-GCU-trnT-CGU_p5 CCAACGCTTTAGTCCACTCA AGGAACGAATCACACTTTTACCA 50.0 39.1 57.8 58.3 720-954 bp 1.0 59.4