Hotspot

rps16-trnQ-UUG

LSC · 6666-7214 · 549 bp

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Pi 0.0637
MarkerSeek score 81.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
549
variable_sites
32
pi
0.0637
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAGCAGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 634-648 bp 1.0 84.1
rps16-trnQ-UUG_p2 TCCACAGCAGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 635-649 bp 1.0 84.0
rps16-trnQ-UUG_p3 GTCCTTCAATTCAAGTCGCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 620-634 bp 1.0 83.4
rps16-trnQ-UUG_p4 GCGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 601-615 bp 1.0 83.4
rps16-trnQ-UUG_p5 ATCCACAGCAGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 636-650 bp 1.0 82.3