Hotspot

ycf1-trnN-GUU

IRa · 134156-134602 · 447 bp

Back to Clematoclethra

Pi 0

MarkerSeek score 48.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 447
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATAGAGTCCGACCACAACGA	AGGATTCGAACCTACGACCA	50.0	50.0	58.2	58.2	525-605 bp	1.0	81.4
ycf1-trnN-GUU_p2	ACCACAACGACCGAATTGAT	AGGATTCGAACCTACGACCA	45.0	50.0	57.8	58.2	515-595 bp	1.0	81.3
ycf1-trnN-GUU_p3	ATAGAGTCCGACCACAACGA	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.2	57.7	533-613 bp	1.0	80.0
ycf1-trnN-GUU_p4	ACCACAACGACCGAATTGAT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.8	57.7	523-603 bp	1.0	80.0
ycf1-trnN-GUU_p5	GACCACAACGACCGAATTGA	AGGATTCGAACCTACGACCA	50.0	50.0	58.5	58.2	516-596 bp	1.0	78.8