Hotspot

rpl32-trnL-UAG

SSC · 116681-117477 · 797 bp

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Pi 0.00502
MarkerSeek score 61.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
797
variable_sites
4
pi
0.00502
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATTGGGCAGCCTTAAAAGCT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 943 bp 1.0 78.9
rpl32-trnL-UAG_p2 ATTGGGCAGCCTTAAAAGCT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 936 bp 1.0 78.4
rpl32-trnL-UAG_p3 ATTGGGCAGCCTTAAAAGCT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 942 bp 1.0 78.3
rpl32-trnL-UAG_p4 ATTGGGCAGCCTTAAAAGCT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 941 bp 1.0 78.3
rpl32-trnL-UAG_p5 AAAGCATATTGGGCAGCCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 950 bp 1.0 78.3