Hotspot

ndhF-rpl32

SSC · 115532-116500 · 969 bp

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Pi 0.00517

MarkerSeek score 61.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 969
variable_sites: 5
pi: 0.00517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAAAAGGTATGATCCACGCA	AGCTTTTAAGGCTGCCCAAT	42.9	45.0	57.9	58.1	1102-1103 bp	1.0	71.5
ndhF-rpl32_p2	ACAAAAGGTATGATCCACGCA	AAGGCTGCCCAATATGCTTT	42.9	45.0	57.9	58.1	1095-1096 bp	1.0	70.9
ndhF-rpl32_p3	ACAAAAGGTATGATCCACGCA	AAAGCTTTTAAGGCTGCCCA	42.9	45.0	57.9	58.3	1104-1105 bp	1.0	69.7
ndhF-rpl32_p4	ACAAAAGGTATGATCCACGCA	AAGCTTTTAAGGCTGCCCAA	42.9	45.0	57.9	58.3	1103-1104 bp	1.0	69.7
ndhF-rpl32_p5	TGTTGCCGTTGGAAAAAGAA	AGCTTTTAAGGCTGCCCAAT	40.0	45.0	56.7	58.1	1160-1161 bp	1.0	69.4