Hotspot

trnN-GUU-ndhF

IRb · 112302-113287 · 986 bp

Back to Clematoclethra

Pi 0.00111

MarkerSeek score 54.90

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 986
variable_sites: 1
pi: 0.00111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GGGGGCGCATCTCTTTTTAT	50.0	50.0	58.2	58.3	1049-1137 bp	1.0	80.4
trnN-GUU-ndhF_p2	CCCCAAGTAGGATTCGAACC	GGGGGCGCATCTCTTTTTAT	55.0	50.0	57.7	58.3	1057-1145 bp	1.0	79.0
trnN-GUU-ndhF_p3	GGATTCGAACCTACGACCAA	GGGGGCGCATCTCTTTTTAT	50.0	50.0	57.3	58.3	1048-1136 bp	1.0	76.3
trnN-GUU-ndhF_p4	TCTCCCCAAGTAGGATTCGA	GGGGGCGCATCTCTTTTTAT	50.0	50.0	57.2	58.3	1060-1148 bp	1.0	74.9
trnN-GUU-ndhF_p5	AGGATTCGAACCTACGACCA	GGGGCGCATCTCTTTTTATC	50.0	50.0	58.2	56.9	1048-1136 bp	1.0	74.3