Hotspot

rps19-rpl2

LSC · 87303-87354 · 52 bp

Back to Clematoclethra

Pi 0.0192

MarkerSeek score 63.60

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 52
variable_sites: 1
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CCGCGACCAGGTTACTATTA	CACAACCCCTTGGGGTTATC	50.0	55.0	56.8	58.2	260 bp	1.0	69.8
rps19-rpl2_p2	CCGCGACCAGGTTACTATTA	TTGGGGTTATCCTGCACTTG	50.0	50.0	56.8	57.8	251 bp	1.0	69.3
rps19-rpl2_p3	CCGCGACCAGGTTACTATTA	GGGTTATCCTGCACTTGGAA	50.0	50.0	56.8	57.5	248 bp	1.0	67.0
rps19-rpl2_p4	CCGCGACCAGGTTACTATTA	CTTGGGGTTATCCTGCACTT	50.0	50.0	56.8	57.5	252 bp	1.0	66.9
rps19-rpl2_p5	CCCGCGACCAGGTTACTATT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.5	58.2	261 bp	1.0	66.8