Hotspot

rps12-psbB

LSC · 72109-75276 · 3168 bp

Back to Clematoclethra

Pi 0.00601

MarkerSeek score 59.10

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 3168
variable_sites: 19
pi: 0.00601
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	GGCCTTGGTGGATGATAAGG	GCTCGACACCCGACTAATTT	55.0	50.0	58.0	58.0	845-851 bp	1.0	81.7
rps12-psbB_p2	TCATGCCTTCTGCCAACTTT	GCTCGACACCCGACTAATTT	45.0	50.0	58.0	58.0	1411-1417 bp	1.0	81.1
rps12-psbB_p3	TTCATGCCTTCTGCCAACTT	GCTCGACACCCGACTAATTT	45.0	50.0	58.0	58.0	1412-1418 bp	1.0	81.1
rps12-psbB_p4	TTTCATGCCTTCTGCCAACT	GCTCGACACCCGACTAATTT	45.0	50.0	58.0	58.0	1413-1419 bp	1.0	81.1
rps12-psbB_p5	ATGCTCCCCTTACCACTTCT	GCTCGACACCCGACTAATTT	50.0	50.0	58.0	58.0	1462-1468 bp	1.0	80.9