Hotspot

rbcL-accD

LSC · 57885-58570 · 686 bp

Back to Clematoclethra

Pi 0.00729

MarkerSeek score 53.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 5
pi: 0.00729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	GACATTTTTCCCCTTTTTCCCC	50.0	45.5	57.9	58.0	787 bp	0.5	43.0
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ACATTTTTCCCCTTTTTCCCC	50.0	42.9	57.9	56.8	786 bp	0.5	41.8
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	GACATTTTTCCCCTTTTTCCCC	55.0	45.5	57.7	58.0	803 bp	0.5	41.5
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	ACATTTTTCCCCTTTTTCCCCT	50.0	40.9	57.9	58.3	786 bp	0.5	40.5
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	ACATTTTTCCCCTTTTTCCCC	55.0	42.9	57.7	56.8	802 bp	0.5	40.4