Hotspot

trnE-UUC-trnT-GGU

LSC · 31314-32129 · 816 bp

Back to Clematoclethra

Pi 0.00368

MarkerSeek score 60.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 3
pi: 0.00368
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	899 bp	1.0	78.4
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	896 bp	1.0	78.2
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	877 bp	1.0	77.5
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	874 bp	1.0	77.3
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	ACGACTTACGCCTTACCATG	50.0	50.0	57.8	57.7	914 bp	1.0	76.6