Hotspot

trnC-GCA-petN

LSC · 28592-29364 · 773 bp

Back to Clematoclethra

Pi 0.00517

MarkerSeek score 53.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 4
pi: 0.00517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTGTATCCGTTTGGCGACAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	883-884 bp	1.0	78.3
trnC-GCA-petN_p2	TTTGTATCCGTTTGGCGACA	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.1	58.2	884-885 bp	1.0	78.2
trnC-GCA-petN_p3	TTGTATCCGTTTGGCGACAT	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	944-945 bp	1.0	76.7
trnC-GCA-petN_p4	TTTGTATCCGTTTGGCGACA	AGAGTCCACTTCTTCCCCAT	45.0	50.0	58.1	57.6	945-946 bp	1.0	76.6
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	847-848 bp	1.0	76.3