Hotspot

rps16-trnQ-UUG

LSC · 6260-7130 · 871 bp

Back to Clematoclethra

Pi 0.00119

MarkerSeek score 62.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 1
pi: 0.00119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.9	58.0	993-1019 bp	1.0	81.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	976-1002 bp	1.0	81.4
rps16-trnQ-UUG_p3	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.2	58.0	994-1020 bp	1.0	80.8
rps16-trnQ-UUG_p4	AACGGATCGTGTCCTTCAAG	TAAGGCACCGGGTTTTGATC	50.0	50.0	57.9	58.2	965-991 bp	1.0	80.6
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCACCGGGTTTTGATC	45.0	50.0	58.1	58.2	948-974 bp	1.0	80.2