Hotspot

ndhG

SSC · 118845-119378 · 534 bp

Back to Clematis

Pi 0.00199

MarkerSeek score 33.20

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 534
variable_sites: 28
pi: 0.00199
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TCTGCTCATGAAAGAAGGCC	AACCCACACACGACCCTATA	50.0	50.0	57.9	58.1	1242-1405 bp	0.9875	90.1
ndhG_p2	CTGCTCATGAAAGAAGGCCA	AACCCACACACGACCCTATA	50.0	50.0	58.2	58.1	1241-1404 bp	0.9875	89.7
ndhG_p3	TCTGCTCATGAAAGAAGGCC	ACCCACACACGACCCTATAT	50.0	50.0	57.9	57.8	1241-1404 bp	0.9875	89.2
ndhG_p4	TCTGCTCATGAAAGAAGGCC	GTCGGTTGCCAATGTCAGTA	50.0	50.0	57.9	58.2	1716-1877 bp	0.9875	89.0
ndhG_p5	TCTGCTCATGAAAGAAGGCC	TACATCAACCCACACACGAC	50.0	50.0	57.9	57.8	1248-1411 bp	0.98125	89.0