Hotspot

ndhD

SSC · 116113-117591 · 1479 bp

Back to Clematis

Pi 0.00386

MarkerSeek score 44.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1479
variable_sites: 104
pi: 0.00386
unique_haplotype_count: 88
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTAATGAAGCGTGGGGATCC	GAGACAACTCGTAGCATGGG	55.0	55.0	58.1	58.1	2143-2235 bp	1.0	90.5
ndhD_p2	CTAATGAAGCGTGGGGATCC	TGCTTCTGCTCCAAGAACAG	55.0	50.0	58.1	58.1	2240-2332 bp	1.0	90.2
ndhD_p3	CTAATGAAGCGTGGGGATCC	TACGGGTTTTCGGGTCAAAG	55.0	50.0	58.1	58.1	1995-2089 bp	1.0	90.1
ndhD_p4	CTAATGAAGCGTGGGGATCC	AGAGATGATACCCTGGGACG	55.0	55.0	58.1	58.0	2279-2371 bp	0.975	90.1
ndhD_p5	GGGAGCAGTATGGGCTAATG	AGAGATGATACCCTGGGACG	55.0	55.0	57.8	58.0	2293-2385 bp	0.975	89.5