Hotspot

ndhG-ndhI

SSC · 119379-119870 · 492 bp

Back to Clematis

Pi 0.0162

MarkerSeek score 71.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 492
variable_sites: 103
pi: 0.0162
unique_haplotype_count: 107
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACCACTCCTAGACTTCCCAC	GTCGGTTGCCAATGTCAGTA	55.0	50.0	58.1	58.2	665-712 bp	0.9875	89.4
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAA	GTCGGTTGCCAATGTCAGTA	45.0	50.0	57.9	58.2	705-752 bp	0.9875	89.4
ndhG-ndhI_p3	CCACTCCTAGACTTCCCACT	GTCGGTTGCCAATGTCAGTA	55.0	50.0	57.8	58.2	664-711 bp	0.9875	88.0
ndhG-ndhI_p4	ACCACTCCTAGACTTCCCAC	TCGGTTGCCAATGTCAGTAA	55.0	45.0	58.1	57.4	664-711 bp	0.9875	86.6
ndhG-ndhI_p5	TCCCAACGAAAAGGCAGAAA	TCGGTTGCCAATGTCAGTAA	45.0	45.0	57.9	57.4	704-751 bp	0.9875	86.6