Hotspot

rpl32-trnL-UAG

SSC · 113879-114662 · 784 bp

Back to Clematis

Pi 0.018
MarkerSeek score 70.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
784
variable_sites
177
pi
0.018
unique_haplotype_count
122
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGATATGAACCAACGAATTCGA AGCGTGTCTACCGATTTCAC 40.9 50.0 57.0 58.0 803-861 bp 0.95 65.7
rpl32-trnL-UAG_p2 TCGATATGAACCAACGAATTCG AGCGTGTCTACCGATTTCAC 40.9 50.0 57.0 58.0 804-862 bp 0.95 65.7
rpl32-trnL-UAG_p3 CGATATGAACCAACGAATTCGA CAGCGTGTCTACCGATTTCA 40.9 50.0 57.0 58.0 804-862 bp 0.95 65.7
rpl32-trnL-UAG_p4 TCGATATGAACCAACGAATTCG CAGCGTGTCTACCGATTTCA 40.9 50.0 57.0 58.0 805-863 bp 0.95 65.7
rpl32-trnL-UAG_p5 CGATATGAACCAACGAATTCGA TCCTAAGAGCAGCGTGTCTA 40.9 50.0 57.0 57.9 813-871 bp 0.95 64.8