Hotspot

ndhF-rpl32

SSC · 112592-113632 · 1041 bp

Back to Clematis

Pi 0.0228

MarkerSeek score 67.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1041
variable_sites: 264
pi: 0.0228
unique_haplotype_count: 139
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGGGGGACGAAAGGTATGA	ATCCCCATCCCACAAAAACC	50.0	50.0	58.0	58.0	1350-1582 bp	0.95625	89.5
ndhF-rpl32_p2	TTGGCAACCGAACCTACAAA	ATCCCCATCCCACAAAAACC	45.0	50.0	57.9	58.0	2021-2253 bp	0.95625	89.4
ndhF-rpl32_p3	ACGAAAGGTATGATCCACGC	ATCCCCATCCCACAAAAACC	50.0	50.0	58.1	58.0	1343-1575 bp	0.95	89.1
ndhF-rpl32_p4	AAGGGGGACGAAAGGTATGA	CGGCATCCAGAACCCAATAA	50.0	50.0	58.0	57.9	1433-1670 bp	0.90625	87.5
ndhF-rpl32_p5	TTGGCAACCGAACCTACAAA	CGGCATCCAGAACCCAATAA	45.0	50.0	57.9	57.9	2104-2341 bp	0.90625	87.4