Hotspot

ycf4-cemA

LSC · 60480-61078 · 599 bp

Back to Clematis

Pi 0.0103

MarkerSeek score 67.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 84
pi: 0.0103
unique_haplotype_count: 95
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAAAGAAATCCACCAGGGCA	45.0	50.0	57.9	58.1	687-735 bp	0.96875	89.5
ycf4-cemA_p2	AATTGAACAAAAGGCTGCGG	GAAAGAAATCCACCAGGGCA	45.0	50.0	57.8	58.1	722-770 bp	0.96875	88.9
ycf4-cemA_p3	AACAAAAGGCTGCGGAATTG	GAAAGAAATCCACCAGGGCA	45.0	50.0	57.8	58.1	717-765 bp	0.9625	88.7
ycf4-cemA_p4	TGCGCGTACCAATTGAAGTA	AGAAAGAAATCCACCAGGGC	45.0	50.0	57.9	57.8	688-736 bp	0.96875	88.3
ycf4-cemA_p5	AACAAAAGGCTGCGGAATTG	AGAAAGAAATCCACCAGGGC	45.0	50.0	57.8	57.8	718-766 bp	0.9625	87.5