Hotspot

atpB-rbcL

LSC · 54361-55223 · 863 bp

Back to Clematis

Pi 0.00974

MarkerSeek score 64.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 110
pi: 0.00974
unique_haplotype_count: 111
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	AGCCAACACTCGCTTTAGTC	55.0	50.0	58.0	58.2	953-1037 bp	0.975	89.0
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	GCCAACACTCGCTTTAGTCT	55.0	50.0	58.0	58.2	952-1036 bp	0.975	89.0
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	TGAAGCCAACACTCGCTTTA	55.0	45.0	58.0	57.8	956-1040 bp	0.975	88.6
atpB-rbcL_p4	CATCCAGTACCGGACCAATG	AAGCCAACACTCGCTTTAGT	55.0	45.0	58.0	57.7	954-1038 bp	0.975	88.6
atpB-rbcL_p5	TTCTTCAAGAGCGGAAACCC	GCCAACACTCGCTTTAGTCT	50.0	50.0	58.1	58.2	906-990 bp	0.975	88.5