Hotspot

trnS-GGA-trnV-UAC

LSC · 50159-51270 · 1112 bp

Back to Clematis

Pi 0.0115

MarkerSeek score 67.00

Primer available yes

Label trnS-GGA-trnV-UAC

Feature Metadata

label_name: trnS-GGA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 162
pi: 0.0115
unique_haplotype_count: 121
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-trnV-UAC_p1	AGGCTTTTGTTTACCGAGGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	964-1214 bp	1.0	89.7
trnS-GGA-trnV-UAC_p2	TTTAGGAGAGATGGCCGAGT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1015-1265 bp	1.0	89.1
trnS-GGA-trnV-UAC_p3	GGCTTTTGTTTACCGAGGGT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	963-1213 bp	1.0	87.3
trnS-GGA-trnV-UAC_p4	AGGCTTTTGTTTACCGAGGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	963-1213 bp	1.0	86.9
trnS-GGA-trnV-UAC_p5	TCTTTAGGAGAGATGGCCGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1017-1267 bp	1.0	86.8