Hotspot

atpH-atpI

LSC · 17385-18488 · 1104 bp

Back to Clematis

Pi 0.00553

MarkerSeek score 68.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1104
variable_sites: 119
pi: 0.00553
unique_haplotype_count: 96
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGGTCCAATAGAAGCCA	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	902-1275 bp	1.0	91.2
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	875-1248 bp	1.0	90.3
atpH-atpI_p3	GATACCTTCTACGGCTTGGC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.1	57.9	944-1317 bp	1.0	90.2
atpH-atpI_p4	CGATACCTTCTACGGCTTGG	ATAGGGGAATCCATGGAGGG	55.0	55.0	57.9	57.9	945-1318 bp	1.0	90.2
atpH-atpI_p5	AACGGAAGCAGCAGAAATCA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.1	57.9	857-1193 bp	0.98125	89.8