Hotspot

psbI-trnS-GCU

LSC · 8559-8644 · 86 bp

Back to Clematis

Pi 0.0301

MarkerSeek score 67.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 11
pi: 0.0301
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.1	58.0	174-184 bp	1.0	81.7
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.1	58.0	175-185 bp	1.0	81.7
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	193-203 bp	1.0	80.8
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	47.6	50.0	57.8	58.0	174-184 bp	1.0	79.4
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	47.6	50.0	57.8	58.0	175-185 bp	1.0	79.4