Hotspot

rps16-trnK-UUU

LSC · 2342-2990 · 649 bp

Back to Clematis

Pi 0.0116

MarkerSeek score 72.70

Primer available yes

Label rps16-trnK-UUU

Feature Metadata

label_name: rps16-trnK-UUU
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 132
pi: 0.0116
unique_haplotype_count: 103
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnK-UUU_p1	GCTCAACCTACGGAAACTGT	TCTACCAACGGTATGGACGA	50.0	50.0	57.8	57.9	783-845 bp	1.0	89.7
rps16-trnK-UUU_p2	GCTCAACCTACGGAAACTGT	AAAGCCGAGTACTCTACCGT	50.0	50.0	57.8	58.2	718-780 bp	1.0	89.3
rps16-trnK-UUU_p3	GCTCAACCTACGGAAACTGT	AAGCCGAGTACTCTACCGTT	50.0	50.0	57.8	58.2	717-779 bp	1.0	89.3
rps16-trnK-UUU_p4	CCTTGAAAAAGGCGCTCAAC	TCTACCAACGGTATGGACGA	50.0	50.0	58.2	57.9	796-858 bp	1.0	89.1
rps16-trnK-UUU_p5	CTTGAAAAAGGCGCTCAACC	TCTACCAACGGTATGGACGA	50.0	50.0	58.2	57.9	795-857 bp	1.0	89.1