Hotspot

rps4-rps16

LSC · 634-1196 · 563 bp

Back to Clematis

Pi 0.0137

MarkerSeek score 73.00

Primer available yes

Label rps4-rps16

Feature Metadata

label_name: rps4-rps16
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 128
pi: 0.0137
unique_haplotype_count: 106
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-rps16_p1	GTAAGTGGGTCGGTTTGGAA	ATTCCTTCAAGTCGCACGTT	50.0	45.0	57.7	58.1	661-710 bp	1.0	89.0
rps4-rps16_p2	GTAAGTGGGTCGGTTTGGAA	AAGTCGCACGTTGCTTTCTA	50.0	45.0	57.7	58.1	653-702 bp	1.0	88.8
rps4-rps16_p3	AAGTGGGTCGGTTTGGAAAT	ATTCCTTCAAGTCGCACGTT	45.0	45.0	57.6	58.1	659-708 bp	1.0	88.0
rps4-rps16_p4	AAGTGGGTCGGTTTGGAAAT	AAGTCGCACGTTGCTTTCTA	45.0	45.0	57.6	58.1	651-700 bp	1.0	87.9
rps4-rps16_p5	AGTAAGTGGGTCGGTTTGGA	ATTCCTTCAAGTCGCACGTT	50.0	45.0	58.6	58.1	662-711 bp	1.0	86.4