Hotspot

ycf3

LSC · 42145-44143 · 1999 bp

Back to Cleistogenes

Pi 0.000618

MarkerSeek score 46.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1999
variable_sites: 4
pi: 0.000618
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	CAACCAAACCGCCCATTTTA	TGAGCTGAGTGGAAAATCCA	45.0	45.0	57.2	56.7	2154-2158 bp	1.0	63.7
ycf3_p2	ACCAAACCGCCCATTTTATG	TGAGCTGAGTGGAAAATCCA	45.0	45.0	57.0	56.7	2152-2156 bp	1.0	61.9
ycf3_p3	AACAACCAAACCGCCCATTT	TGAGCTGAGTGGAAAATCCA	45.0	45.0	59.2	56.7	2156-2160 bp	1.0	60.9
ycf3_p4	ACAACCAAACCGCCCATTTT	TGAGCTGAGTGGAAAATCCA	45.0	45.0	59.2	56.7	2155-2159 bp	1.0	60.9
ycf3_p5	AACCAAACCGCCCATTTTATG	TGAGCTGAGTGGAAAATCCA	42.9	45.0	57.7	56.7	2153-2157 bp	1.0	59.6