Hotspot

petD

LSC · 72783-74012 · 1230 bp

Back to Cleistogenes

Pi 0.000739

MarkerSeek score 49.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1230
variable_sites: 5
pi: 0.000739
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACGAAAGAAAATAAGGCGGA	GGGACTTCACGGTTGAATGA	40.0	50.0	55.4	57.8	1271-1289 bp	1.0	59.0
petD_p2	ACGAAAGAAAATAAGGCGGAT	GGGACTTCACGGTTGAATGA	38.1	50.0	56.0	57.8	1271-1289 bp	1.0	55.6
petD_p3	ACGAAAGAAAATAAGGCGGA	GGGGACTTCACGGTTGAATG	40.0	55.0	55.4	58.8	1272-1290 bp	1.0	53.8
petD_p4	ACGAAAGAAAATAAGGCGGATT	GGGACTTCACGGTTGAATGA	36.4	50.0	56.7	57.8	1271-1289 bp	1.0	53.5
petD_p5	ACGAAAGAAAATAAGGCGGA	AGGGGACTTCACGGTTGAAT	40.0	50.0	55.4	58.9	1273-1291 bp	1.0	53.0