Hotspot

petA-psbJ

LSC · 60427-61355 · 929 bp

Back to Cleistogenes

Pi 0.00231

MarkerSeek score 50.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 929
variable_sites: 7
pi: 0.00231
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.7	58.1	1047-1062 bp	1.0	75.3
petA-psbJ_p2	GATCCATTACGCGTCCAAGG	TCCGGATTGGGTTCATCTCT	55.0	50.0	58.8	58.1	1058-1073 bp	1.0	74.5
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	CGTATTCCGGATTGGGTTCA	50.0	50.0	58.7	57.7	1052-1067 bp	1.0	73.7
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GTTCGTATTCCGGATTGGGT	50.0	50.0	58.7	57.7	1055-1070 bp	1.0	73.7
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCCGGATTGGG	50.0	55.0	58.7	58.4	1056-1071 bp	1.0	73.0